GPC1 Knockout cell line (BxPC-3)

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GPC1 Knockout cell line (BxPC-3) Order

GPC1 Knockout cell line (BxPC-3)
Catalog#
YKO-H983
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
GPC1 Knockout cell line (BxPC-3)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
GPC1 Knockout cell line（BxPC-3）
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
GPC1
Gene ID
2817
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Accession
CVCL_D8F1(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 3 (based on transcript GPC1-201)
Validation: PCR+Sanger Sequencing
GPC1 Knockout cell line (BxPC-3) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:BxPC-3		 STR Info (Cell bank)
Cell Line:BX-PC3
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11111111
D7S820 10131013
D16S539 911911
VWA 14181418
TH01 9999
AMEL XXXX
TPOX 8888
CSF1PO 13131313
D12S391 19.320
FGA 2021
D2S1338 1719
D21S11 2929
D18S51 1212
D8S1179 1313
D3S1358 1416
D6S1043 1212
PENTAE 1214
D19S433 1313
PENTAD 1414
D1S1656 1617.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the GPC1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the BxPC-3 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, GPC1 gene knockout GPC1 Knockout cell line（BxPC-3） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
GPC1 Knockout cell line (BxPC-3)
GPC1 Knockout cell line (BxPC-3)
Catalog#
YKO-H983
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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